[Coverslip assisted primary tissue culture for human lens epithelial cells in vitro].
| Autor: | Peng R; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China., Wu M, Ye S |
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| Jazyk: | čínština |
| Zdroj: | Yan ke xue bao = Eye science [Yan Ke Xue Bao] 2008 Mar; Vol. 24 (1), pp. 23-6. |
| Abstrakt: | Purpose: To set up an easy procedure of tissue culture for human lens epithelial cells in vitro and to observe the biological characteristics. Methods: Capsules from embryo of 20 weeks, eye bank of Zhongshan Ophthalmic Centre and patients with cataract were spread on culture utensil. 10 microL of 10% DMEM medium was added and a piece of coverslip was lay to prevent crimp. Then the capsules were cultured under 37 degrees C after adding enough medium. Capsules from the same source were cultured by traditional tissue culture method. Expressions of 13 crystallin between primary tissue culture cells and SRA01/04 cell line were compared by western blotting. Results: With coverslip assisted, the cells could be observed proliferated and migrated from the edge of embryo capsule 2 days later, and for capsules from eye bank and age-related cataract patients, the interval time was 3 to 4 days. By traditional tissue culture method, the interval time of embryo capsule was 3 to 4 days, and for capsules from eye bank and age-related cataract patients, the interval time was the same. And capsules floated sometimes. Conclusions: By coverslip assisted primary tissue culture human lens epithelial cells could grow faster and easier, and the method is worthy to be spread in research of lens diseases. |
| Databáze: | MEDLINE |
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