| Autor: |
de Castro Oliveira JV; Departamento de Microbiologia, Laboratório de Evolução Molecular e Bioinformática, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil., de Melo FL, Romano CM, Iamarino A, Rizzi TS, Yeda FP, Hársi CM, Wolff JL, de Andrade Zanotto PM |
| Jazyk: |
angličtina |
| Zdroj: |
Virus genes [Virus Genes] 2008 Oct; Vol. 37 (2), pp. 177-84. Date of Electronic Publication: 2008 Jul 22. |
| DOI: |
10.1007/s11262-008-0253-x |
| Abstrakt: |
ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of <1 A with other PARP available structures. The 5' end of ORF 31 mRNA was located at the first nucleotide of a CATT motif at position -27. Using real-time PCR we detected transcripts at 3 h post-infection (p.i.) increasing until 24 h p.i., which coincides with the onset of DNA replication, suggestive of a possible role in DNA metabolism. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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