Ultrastructure of islet microcirculation, pericytes and the islet exocrine interface in the HIP rat model of diabetes.
| Autor: | Hayden MR; Department of Internal Medicine, University of Missouri School of Medicine, Columbia, Missouri 65212, USA. mrh29@usmo.com, Karuparthi PR, Habibi J, Lastra G, Patel K, Wasekar C, Manrique CM, Ozerdem U, Stas S, Sowers JR |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2008 Sep; Vol. 233 (9), pp. 1109-23. Date of Electronic Publication: 2008 Jul 18. |
| DOI: | 10.3181/0709-RM-251 |
| Abstrakt: | Context: The transgenic human islet amyloid polypeptide (HIP) rat model of type 2 diabetes mellitus (T2DM) parallels the functional and structural changes in human islets with T2DM. Objective: The transmission electron microscope (TEM) was utilized to observe the ultrastructural changes in islet microcirculation. Methods: Pancreatic tissue from male Sprague Dawley rats (2, 4, 8, 14 months) were used as controls (SDC) and compared to the 2-, 4-, 8- and 14-month-old HIP rat models. Results: The 2-month-old HIP model demonstrated no islet or microcirculation remodeling changes when compared to the SDC models. The 4-month-old HIP model demonstrated significant pericapillary amyloid deposition and diminution of pericyte foot processes as compared to the SDC models. The 8-month-old model demonstrated extensive islet amyloid deposition associated with pericyte and beta-cell apoptosis when compared with SDC. The 14-month-old HIP model demonstrated a marked reduction of beta-cells and intra-islet capillaries with near complete replacement of islets by amyloidoses. Increased cellularity in the region of the islet exocrine interface was noted in the 4- to 14-month-old HIP models as compared to SDC. In contrast to intra-islet capillary rarefaction there was noticeable angiogenesis in the islet exocrine interface. Pericytes seemed to be closely associated with collagenosis, intra-islet adipogenesis and angiogenesis in the islet exocrine interface. Conclusion: The above novel findings regarding the microcirculation and pericytes could assist researchers and clinicians in a better morphological understanding of T2DM and lead to new strategies for prevention and treatment of T2DM. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|