Leukotriene B4-loaded microspheres: a new therapeutic strategy to modulate cell activation.

Autor: Nicolete R; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av, do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brasil. nicolete@fcfrp.usp.br; Cristina Rius, Rius C, Piqueras L, Jose PJ, Sorgi CA, Soares EG, Sanz MJ, Faccioli LH
Jazyk: angličtina
Zdroj: BMC immunology [BMC Immunol] 2008 Jul 15; Vol. 9, pp. 36. Date of Electronic Publication: 2008 Jul 15.
DOI: 10.1186/1471-2172-9-36
Abstrakt: Background: Leukotriene B4 (LTB4) is a potent inflammatory mediator that also stimulates the immune response. In addition, it promotes polymorphonuclear leukocyte phagocytosis, chemotaxis, chemokinesis and modulates cytokines release. Regarding chemical instability of the leukotriene molecule, in the present study we assessed the immunomodulatory activities conferred by LTB4 released from microspheres (MS). A previous oil-in-water emulsion solvent extraction-evaporation method was chosen to prepare LTB4-loaded MS.
Results: In the mice cremasteric microcirculation, intraescrotal injection of 0.1 ml of LTB4-loaded MS provoked significant increases in leukocyte rolling flux, adhesion and emigration besides significant decreases in the leukocyte rolling velocity. LTB4-loaded MS also increase peroxisome proliferator-activated receptor-alpha (PPARalpha) expression by murine peritoneal macrophages and stimulate them to generate nitrite levels. Monocyte chemoattractant protein-1 (MCP-1) and nitric oxide (NO) productions were also increased when human umbilical vein and artery endothelial cells (HUVECs and HUAECs, respectively) were stimulated with LTB4-loaded MS.
Conclusion: LTB4-loaded MS preserve the biological activity of the encapsulated mediator indicating their use as a new strategy to modulate cell activation, especially in the innate immune response.
Databáze: MEDLINE
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