[Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC].

Autor:	He FY; Pharmacogenetic Research Institute, Central South University, Changsha 410078, China. pharmsharking@tom.com, Zhou HH, Luo JY, Deng KW, Fu J, Pi FJ, Wu DZ, Dai RW
Jazyk:	čínština
Zdroj:	Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2008 Mar; Vol. 33 (6), pp. 642-8.
Abstrakt:	Objective: To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC. Method: The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL. Result: The mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28.31 J(1/2) cm(-3/2) in accordance with 28.84 J(1/2) cm(-3/2) reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39.70 J(1/2) cm(-3/2), 39.08 J(1/2) cm(-3/2), 38.37 J(1/2) cm(-3/2), 36.42 J(1/2) cm(-3/2) respectively. Conclusion: The retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine.
Databáze:	MEDLINE
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