A physical map of human Alu repeats cleavage by restriction endonucleases.
| Autor: | Abdurashitov MA; SibEnzyme, 2/12 Ak, Timakov Str, 630117 Novosibirsk, Russia. abd@sibenzyme.ru, Tomilov VN, Chernukhin VA, Degtyarev SKh |
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| Jazyk: | angličtina |
| Zdroj: | BMC genomics [BMC Genomics] 2008 Jun 26; Vol. 9, pp. 305. Date of Electronic Publication: 2008 Jun 26. |
| DOI: | 10.1186/1471-2164-9-305 |
| Abstrakt: | Background: Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases. We have proposed a method of restriction analysis of Alu repeats sequences in silico. Results: Simple software to analyze Alu repeats database has been suggested and Alu repeats digestion patterns for several restriction enzymes' recognition sites have been constructed. Restriction maps of Alu repeats cleavage for corresponding restriction enzymes have been calculated and plotted. Theoretical data have been compared with experimental results on DNA hydrolysis with restriction enzymes, which we obtained earlier. Conclusion: Alu repeats digestions provide the main contribution to the patterns of human chromosomal DNA cleavage. This corresponds to the experimental data on total human DNA hydrolysis with restriction enzymes. |
| Databáze: | MEDLINE |
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