| Autor: |
Besselink H; BioDetection Systems BV, Kruislaan 406, 1098 SM, Amsterdam, The Netherlands. harrie.besselink@bds.nl, Nixon E, McHugh B, Rimkus G, Klungsøyr J, Leonards P, De Boer J, Brouwer A |
| Jazyk: |
angličtina |
| Zdroj: |
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2008 Aug; Vol. 46 (8), pp. 2629-38. Date of Electronic Publication: 2008 May 10. |
| DOI: |
10.1016/j.fct.2008.04.039 |
| Abstrakt: |
In this study the potential impact of food chain-based biotransformation and physico-chemical weathering of toxaphene on its tumour promoting potential was investigated in vitro and in vivo. Human exposure to toxaphene is mainly through consumption of contaminated fish, therefore fish-borne residues of toxaphene (cod liver extract, CLE) were prepared by exposing cod to technical toxaphene (TT) for 63 days. UV-irradiated toxaphene (uvT) was included to represent a physico-chemical weathered toxaphene mixture. In vitro, TT, uvT and CLE all showed a dose- and time-dependent inhibition of gap junctional intercellular communication (GJIC) with a relative potency of CLE>TT=uvT. Tumour promoting potency was further studied in vivo in a medium term two-stage initiation/promotion bioassay in female Sprague-Dawley rats, using an increase in altered hepatic foci positive for glutathione-S-transferase-P (AHF-GST-P) as read out. No increase in AHF-GST-P occurred following exposure to either TT, uvT, or CLE, except for the positive control group (2,3,7,8-TCDD). Based on this study the no observed adverse effect level (NOAEL) for tumour promoting potency is at least 12.5mg/kg/week, or higher for CLE. Considering current human exposure levels in Europe it is doubtful that consumption of fish at current levels of toxaphene contamination give rise to human health risk. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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