Usefulness of Sau-PCR for molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia which occurred in a local hospital in Guangzhou, China.

Autor: Yan H; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China., Shi L, Alam MJ, Li L, Yang L, Yamasaki S
Jazyk: angličtina
Zdroj: Microbiology and immunology [Microbiol Immunol] 2008 May; Vol. 52 (5), pp. 283-6.
DOI: 10.1111/j.1348-0421.2008.00035.x
Abstrakt: The aim of the present study was to determine the source of nosocomial outbreak due to Burkholderia cepacia by molecular techniques. A total of 11 B. cepacia strains were isolated; nine from blood and one from sputum of patients without cystic fibrosis, and one from reverse osmosis water at a local hospital in Guangzhou, China. Analyses of 11 strains by the Sau-PCR assay and pulsed-field gel electrophoresis revealed that nine strains obtained from the blood of outpatients in a hemodialysis unit and one strain from reverse osmosis water had identical DNA profiles, indicating that the reverse osmosis water supply could be a source of infection.
Databáze: MEDLINE