| Autor: |
Meged R; Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel., Dym O, Sussman JL |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2008; Vol. 426, pp. 411-8. |
| DOI: |
10.1007/978-1-60327-058-8_27 |
| Abstrakt: |
Most high throughput structural proteomics centers use the sitting-drop method to obtain diffracting crystals for three-dimensional (3D) structure determination of biological macromolecules by x-ray crystallography. Although several robotic systems are available for dispensing the initial sitting-drop screening conditions, generally they are not used for optimization of crystallization conditions. This chapter describes a protocol for such automated systems, which permits easy construction of pH optimization grids using any desired fixed buffer set with varying ionic strengths directly dispensed into the crystallization plate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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