| Autor: |
Morozov SY; Department of Virology, Moscow State University, USSR., Miroshnichenko NA, Solovyev AG, Zelenina DA, Fedorkin ON, Lukasheva LI, Grachev SA, Chernov BK |
| Jazyk: |
angličtina |
| Zdroj: |
Virology [Virology] 1991 Aug; Vol. 183 (2), pp. 782-5. |
| DOI: |
10.1016/0042-6822(91)91011-5 |
| Abstrakt: |
Two double-stranded DNA copies of the genes potentially coding for the 7-kDa proteins of potato virus M (PVM) and potato virus S (PVS) were synthesized and cloned into T7 transcription vectors. Cell-free translation of the corresponding monocistronic transcripts yielded in both cases a single protein of approximately 7-8 kDa that contains a highly hydrophobic N-terminal segment. To analyze their membrane-binding potential, both proteins were synthesized in the membrane-enriched Krebs-2 extract. It was found that the smooth membrane fraction was enriched in the carlavirus 7-kDa proteins. The primary and predicted secondary structures of their N-terminal hydrophobic segments suggest that the latter can function as signals for translocation into the rough endoplasmic reticulum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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