| Autor: |
Moriyama EH; Division of Biophysics and Bioimaging Ontario Cancer Institute/University of Toronto, 610 University Avenue 7-417, Toronto, Ontario M5G 2M9, Canada., Niedre MJ, Jarvi MT, Mocanu JD, Moriyama Y, Subarsky P, Li B, Lilge LD, Wilson BC |
| Jazyk: |
angličtina |
| Zdroj: |
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2008 Jun; Vol. 7 (6), pp. 675-80. Date of Electronic Publication: 2008 Apr 08. |
| DOI: |
10.1039/b719231b |
| Abstrakt: |
Firefly luciferase catalyzes the emission of light from luciferin in the presence of oxygen and adenosine triphosphate. This bioluminescence is commonly employed in imaging mode to monitor tumor growth and treatment responses in vivo. A potential concern is that, since solid tumors are often hypoxic, either constitutively and/or as a result of treatment, the oxygen available for the bioluminescence reaction could be reduced to limiting levels, leading to underestimation of the actual number of luciferase-labeled cells during in vivo experiments. We present studies of the oxygen dependence of bioluminescence in vitro in rat 9 L gliosarcoma cells tagged with the firefly luciferase gene (9L(luc)). We demonstrate that the bioluminescence signal decreases at pO(2)
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| Databáze: |
MEDLINE |
| Externí odkaz: |
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