Measurement of functional fibrinogen levels using the Thrombelastograph.
| Autor: | Carroll RC; Department of Anesthesiology, University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Knoxville, TN 37920, USA. rccarrol1@mc.utmck.edu |
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| Jazyk: | angličtina |
| Zdroj: | Journal of clinical anesthesia [J Clin Anesth] 2008 May; Vol. 20 (3), pp. 186-90. |
| DOI: | 10.1016/j.jclinane.2007.09.017 |
| Abstrakt: | Study Objective: To validate a Thromboelastograph (Haemoscope Corporation, Niles, IL) assay for functional fibrinogen. Design: Correlation study of the Thromboelastograph assay with two conventional fibrinogen assays by the standard Clauss method. Setting: Research laboratory of a university medical center. Participants and Interventions: Blood samples were obtained from 19 healthy volunteers. Measurement and Main Results: Thromboelastograph assays, using heparinized whole blood from 19 healthy donors, indicated that reptilase-XIIIa mixture (Activatorf)-generated clot shear elasticity in dynes per square centimeter (Gf) correlated with fibrinogen (mg/dL). Blood from four donors was used to define the contribution of hematocrit (Hct) to Gf by titration with platelet-rich plasma. The Gf versus Hct gave linear correlations (r2 = 0.746) with Gf = 1258 - 17.8 x % Hct. A commercial collection of 19 normal, 10 borderline, and one deficient for functional fibrinogen-citrated plasmas was assayed for Gf after recalcification using Activatorf. Of the 30 plasma samples, four were from factor X- or factor VII-deficient donors and one was from a coumadin-treated donor. There was a linear correlation of Activatorf Gf with functional fibrinogen (r2 = 0.940) with Gf = -730 + 9.21 x fibrinogen (mg/dL). Conclusion: Thrombelastography with Activatorf may be used to determine fibrinogen levels in whole blood. |
| Databáze: | MEDLINE |
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