| Autor: |
de Lima CJ; Universidade Federal de Uberlândia/FEQ, Caixa Postal 593, 38400-902, Uberlândia, MG, Brazil., França FP, Sérvulo EF, Resende MM, Cardoso VL |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2007 Apr; Vol. 137-140 (1-12), pp. 463-70. |
| DOI: |
10.1007/s12010-007-9072-0 |
| Abstrakt: |
In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite experimental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 +/- 1 degrees C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH(4)NO(3), and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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