A strategy for detecting chromosome-specific rearrangements in rye.

Autor: Alvarez E, Alonso-Blanco C, Suárez RG, Ferreira JJ, Roca A, Giraldez R
Jazyk: angličtina
Zdroj: Genome [Genome] 1997 Aug; Vol. 40 (4), pp. 451-7.
DOI: 10.1139/g97-060
Abstrakt: To obtain translocations involving specific chromosomes in rye, a line in which chromosome 1R has large C-bands on its two telomeres but which lacks C-bands (or has very small ones) on the telomeres of the remaining chromosomes was used. About 6% of the plants produced using pollen from irradiated (1.2 krad (1 rad = 10 mGy)) spikes of this line possessed structural changes involving the labeled chromosome. These aberrations included translocations, ring chromosomes, isochromosomes, and telocentrics. It is concluded (i) that all nonlabeled chromosomes have the same probability of participating in reciprocal translocations with the labeled chromosome, 1R, and (ii) that most induced reciprocal translocations involved exchanges of chromosome segments of approximately equal length. The use of lines having the appropriate combination of telomeric C-bands improves the efficiency of obtaining reciprocal translocations involving specific chromosomes that could be used in the construction of detailed physical maps.
Databáze: MEDLINE