Autor: |
Gately MK; Hoffmann-La Roche Inc, Nutley, New Jersey., Chizzonite R; Hoffmann-La Roche Inc, Nutley, New Jersey., Presky DH; Hoffmann-La Roche Inc, Nutley, New Jersey. |
Jazyk: |
angličtina |
Zdroj: |
Current protocols in immunology [Curr Protoc Immunol] 2001 May; Vol. Chapter 6, pp. 6.16.1-6.16.15. |
DOI: |
10.1002/0471142735.im0616s15 |
Abstrakt: |
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine. |
Databáze: |
MEDLINE |
Externí odkaz: |
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