Measurement of human and mouse interleukin-12.

Autor: Gately MK; Hoffmann-La Roche Inc, Nutley, New Jersey., Chizzonite R; Hoffmann-La Roche Inc, Nutley, New Jersey., Presky DH; Hoffmann-La Roche Inc, Nutley, New Jersey.
Jazyk: angličtina
Zdroj: Current protocols in immunology [Curr Protoc Immunol] 2001 May; Vol. Chapter 6, pp. 6.16.1-6.16.15.
DOI: 10.1002/0471142735.im0616s15
Abstrakt: This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.
Databáze: MEDLINE