| Autor: |
Dedavid e Silva LA; Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Lopes FC, Silveira ST, Brandelli A |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2009 Feb; Vol. 152 (2), pp. 295-305. Date of Electronic Publication: 2008 Apr 22. |
| DOI: |
10.1007/s12010-008-8190-7 |
| Abstrakt: |
The production of cellulolytic enzymes by the fungus Aspergillus phoenicis was investigated. Grape waste from the winemaking industry was chosen as the growth substrate among several agro-industrial byproducts. A 2 x 2 central composite design was performed, utilizing the amount of grape waste and peptone as independent variables. The fungus was cultivated in submerged fermentation at 30 degrees C and 120 rpm for 120 h, and the activities of total cellulases, endoglucanases, and beta-glucosidases were measured. Total cellulases were positively influenced by the linear increase of peptone concentration and decrease at axial concentrations of grape waste and peptone. Maximum activity of endoglucanase was observed by a linear increase of both grape waste and peptone concentrations. Concentrations of grape waste between 5 and 15 g/L had a positive effect on the production of beta-glucosidase; peptone had no significant effects. The optimum production of the three cellulolytic activities was observed at values near the central point. A. phoenicis has the potential for the production of cellulases utilizing grape waste as the growth substrate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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