| Autor: |
Arráez-Román D; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain., Zurek G, Bässmann C, Segura-Carretero A, Fernández-Gutiérrez A |
| Jazyk: |
angličtina |
| Zdroj: |
Electrophoresis [Electrophoresis] 2008 May; Vol. 29 (10), pp. 2112-6. |
| DOI: |
10.1002/elps.200700468 |
| Abstrakt: |
This short communication describes the characterization of seven tropane alkaloid compounds in Atropa belladonna L. Thus a rapid and easy CE-electrospray interface (ESI)-TOF-MS procedure is developed to analyze these compounds in a pharmaceutical preparations of A. belladonna L. leaf extract. Optimum electrophoretic separation is obtained using an alkaline solution of 60 mM ammonium acetate at pH 8.5 containing 5% isopropanol. Under the optimum CE-ESI-TOF-MS conditions several important compounds such as tropine, belladonnine, norhyoscyamine, apoatropine, hyoscyamine, 6beta-hydroxyhyoscyamine, and scopolamine have been simultaneously identified from A. belladonna L. CE-ESI-IT-MS has been used to discriminate the putative presence of littorine. The sensitivity, together with mass accuracy and true isotopic pattern of the TOF-MS, allowed the identification of a broad series of tropane alkaloid compounds present in pharmaceutical preparations of A. belladonna L. leaf extract. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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