| Autor: |
Wickstrom E; Department of Chemistry, University of South Florida, Tampa 33620., Bacon TA, Wickstrom EL, Werking CM, Palmiter RD, Brinster RL, Sandgren EP |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleic acids symposium series [Nucleic Acids Symp Ser] 1991 (24), pp. 151-4. |
| Abstrakt: |
In transgenic mice bearing a murine immunoglobulin enhancer/c-myc fusion transgene (E mu-myc), it was found that methylphosphonates do not induce acute toxicity following intravenous administration of a 300 nmol dose. In addition, recovery of methylphosphonates from the blood plasma of treated mice indicated that the oligomers remained intact up to 3 hours, while their concentrations decreased rapidly for the first hour, then slowly decreased over the next two hours. Finally, methylphosphonate oligomers targeted against c-myc mRNA inhibited production of c-myc p65 protein in peripheral and splenic lymphocytes, relative to a scrambled sequence oligomer, 4 hours after injection of a 300 nmol dose, as indicated by immunofluorescence of fixed cells stained with an anti-c-myc antiserum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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