Optimum diagnostic assay and clinical specimen for routine rotavirus surveillance.

Autor: Stockman LJ; National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Lstockman@cdc.gov, Staat MA, Holloway M, Bernstein DI, Kerin T, Hull J, Yee E, Gentsch J, Parashar UD
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2008 May; Vol. 46 (5), pp. 1842-3. Date of Electronic Publication: 2008 Mar 19.
DOI: 10.1128/JCM.02329-07
Abstrakt: Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.
Databáze: MEDLINE