| Autor: |
Codlin S; MRC Laboratory for Molecular Cell Biology, University College London, WC1E 6BT London, UK., Haines RL, Mole SE |
| Jazyk: |
angličtina |
| Zdroj: |
Traffic (Copenhagen, Denmark) [Traffic] 2008 Jun; Vol. 9 (6), pp. 936-50. Date of Electronic Publication: 2008 Mar 10. |
| DOI: |
10.1111/j.1600-0854.2008.00735.x |
| Abstrakt: |
btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37 degrees C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol-rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe, sterol spreading is linked to defective formation/polarization of F-actin patches and disruption of endocytosis and that these processes are aberrant in btn1Delta cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37 degrees C and is retained in actin-dependent endomembrane structures near the cell poles or septum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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