| Autor: |
Fortin DL; Department of Molecular and Cell Biology, University of California Berkeley, 121 Life Sciences Addition, Berkeley, California 94720, USA., Banghart MR, Dunn TW, Borges K, Wagenaar DA, Gaudry Q, Karakossian MH, Otis TS, Kristan WB, Trauner D, Kramer RH |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2008 Apr; Vol. 5 (4), pp. 331-8. Date of Electronic Publication: 2008 Mar 02. |
| DOI: |
10.1038/nmeth.1187 |
| Abstrakt: |
Light-activated ion channels provide a precise and noninvasive optical means for controlling action potential firing, but the genes encoding these channels must first be delivered and expressed in target cells. Here we describe a method for bestowing light sensitivity onto endogenous ion channels that does not rely on exogenous gene expression. The method uses a synthetic photoisomerizable small molecule, or photoswitchable affinity label (PAL), that specifically targets K+ channels. PALs contain a reactive electrophile, enabling covalent attachment of the photoswitch to naturally occurring nucleophiles in K+ channels. Ion flow through PAL-modified channels is turned on or off by photoisomerizing PAL with different wavelengths of light. We showed that PAL treatment confers light sensitivity onto endogenous K+ channels in isolated rat neurons and in intact neural structures from rat and leech, allowing rapid optical regulation of excitability without genetic modification. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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