| Autor: |
Xue ZT; Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark., Larsen K, Jochimsen BU |
| Jazyk: |
angličtina |
| Zdroj: |
Plant molecular biology [Plant Mol Biol] 1991 May; Vol. 16 (5), pp. 899-906. |
| DOI: |
10.1007/BF00015081 |
| Abstrakt: |
The effect of lowering oxygen concentration on the expression of nodulin genes in soybean callus tissue devoid of the microsymbiont has been examined. Poly(A)+ RNA was isolated from tissue cultivated in 4% oxygen and in normal atmosphere. Quantitative mRNA hybridization experiments using nodule-specific uricase (Nodulin-35) and sucrose synthase (Nodulin-100) cDNA probes confirmed that the synthesis of the uricase and sucrose synthase is controlled by oxygen at the mRNA level. The steady-state levels of uricase and sucrose synthase mRNA increased significantly (5-6- and 4-fold respectively) when the callus tissue was incubated at reduced oxygen concentration. Concomitant with the increase in mRNA level a 6-fold increase in specific activity of sucrose synthase was observed. Two messengers representing poly-ubiquitin precursors also responded to lowering the oxygen concentration. The increase was about 5-fold at 4% oxygen. No expression at atmospheric oxygen or in response to low oxygen was observed when using cDNA probes for other nodulin genes such as leghemoglobin c3, nodulin-22 and nodulin-44. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink