| Autor: |
Krishnakumar R; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Gamble MJ, Frizzell KM, Berrocal JG, Kininis M, Kraus WL |
| Jazyk: |
angličtina |
| Zdroj: |
Science (New York, N.Y.) [Science] 2008 Feb 08; Vol. 319 (5864), pp. 819-21. |
| DOI: |
10.1126/science.1149250 |
| Abstrakt: |
Nucleosome-binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene-specific approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II-transcribed promoters. PARP-1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP-1 acts to exclude H1 from a subset of PARP-1-stimulated promoters, suggesting a functional interplay between PARP-1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP-1 have similar nucleosome-binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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