| Autor: |
Moore MS; Baylor College of Medicine, Houston, Texas, USA., Schwoebel ED |
| Jazyk: |
angličtina |
| Zdroj: |
Current protocols in cell biology [Curr Protoc Cell Biol] 2001 May; Vol. Chapter 11, pp. Unit 11.7. |
| DOI: |
10.1002/0471143030.cb1107s05 |
| Abstrakt: |
Import of proteins into the nucleus of a cell is a complex process that can be reconstituted in vitro. Digitonin-permeabilized cells are washed free of cytosolic factors to provide competent nuclei. Cytosolic factors for import are provided by an extract of Xenopus ovarian cells. Fluorochrome-conjugated probes, cloned proteins fused to green fluorescent protein, or antibodies to the protein of interest are used to visualize nuclear import. The system can also be used to identify nuclear localization sequences (NLS) of imported proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text