| Autor: |
O'Hern PA; Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208., Goldberg E |
| Jazyk: |
angličtina |
| Zdroj: |
Protein expression and purification [Protein Expr Purif] 1991 Feb; Vol. 2 (1), pp. 59-62. |
| DOI: |
10.1016/1046-5928(91)90010-g |
| Abstrakt: |
Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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