| Autor: |
Huyghe A; Genomic Research Laboratory, Infectious Diseases Service, University of Geneva Hospitals, Micheli-du-Crest 24, 1211 Geneva 14, Geneva, Switzerland. antoine.huyghe@genomic.ch, Francois P, Charbonnier Y, Tangomo-Bento M, Bonetti EJ, Paster BJ, Bolivar I, Baratti-Mayer D, Pittet D, Schrenzel J |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2008 Mar; Vol. 74 (6), pp. 1876-85. Date of Electronic Publication: 2008 Jan 18. |
| DOI: |
10.1128/AEM.01722-07 |
| Abstrakt: |
Assessing bacterial flora composition appears to be of increasing importance to fields as diverse as physiology, development, medicine, epidemiology, the environment, and the food industry. We report here the development and validation of an original microarray strategy that allows analysis of the phylogenic composition of complex bacterial mixtures. The microarray contains approximately 9,500 feature elements targeting 16S rRNA gene-specific regions. Probe design was performed by selecting oligonucleotide sequences specific to each node of the seven levels of the bacterial phylogenetic tree (domain, phylum, class, order, family, genus, and species). This approach, based on sequence information, allows analysis of the bacterial contents of complex bacterial mixtures to detect both known and unknown microorganisms. The presence of unknown organisms can be suspected and mapped on the phylogenetic tree, indicating where to refine analysis. Initial proof-of-concept experiments were performed on oral bacterial communities. Our results show that this hierarchical approach can reveal minor changes (
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| Databáze: |
MEDLINE |
| Externí odkaz: |
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