Buffering capacity of bovine vitreous.
| Autor: | Conway MD; Department of Ophthalmology, Tulane University Health Sciences Center, New Orleans, Louisiana, USA. ccole@eyes.arizona.edu, Jermak CM, Peyman GA, Swanson HT, Blake DA |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Retina (Philadelphia, Pa.) [Retina] 2008 Jan; Vol. 28 (1), pp. 150-3. |
| DOI: | 10.1097/IAE.0b013e31806dafb7 |
| Abstrakt: | Purpose: To measure the buffering capacity of bovine vitreous to HCl, NaOH, and triamcinolone acetonide. Methods: Vitreous was dissected from 10 bovine globes, homogenized, and divided into 5.0-mL aliquots. The carbonate/bicarbonate buffer of the vitreous was maintained by performing all subsequent titrations under an atmosphere of 5% CO2/95% air. The pH was monitored during the addition of 0.1 N HCl, 0.1 N NaOH, or 40 mg/mL triamcinolone acetonide to the vitreous sample; 0.9% NaCl was used as control. Results: The buffering capacity of bovine vitreous to HCl was 10 times greater than that of 0.9% NaCl. The buffering capacity of vitreous to NaOH was four times greater than that of 0.9% NaCl. Bovine vitreous was able to resist pH changes from addition of triamcinolone acetonide at levels 20 times greater than commonly injected intravitreally. Conclusion: Vitreous had a greater buffering capacity than 0.9% NaCl, indicating a greater capacity to remain stable when acidic or alkaline pharmaceuticals are injected in microliter amounts. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|