| Autor: |
Anikeeva NN, Vinogradova TV, Dement'eva ES, Ibragimov AR |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 1991 Sep; Vol. 17 (9), pp. 1223-8. |
| Abstrakt: |
A human interleukin-2 (IL-2) gene was isolated from genomic DNA library. The isolated gene with 5'- and 3'-flanking sequences of various lengths was inserted into plasmids derived from the retroviral vector pPSneo. The recombinant plasmids were transfected into myeloma X63Ag8-653 cells. The transfected cells, harbouring the IL-2 gene with the shortened (to position -165) or totally deleted 5'-flanking sequence, constitutively expressed biologically active IL-2. Deletion of 3'-flanking region on did not affect the IL-2 expression. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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