| Autor: |
Ebert AD; The Waisman Center and Department of Anatomy, University of Wisconsin Madison, Madison, WI 53705-2280, USA. ebert@waisman.wisc.edu, Beres AJ, Barber AE, Svendsen CN |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental neurology [Exp Neurol] 2008 Jan; Vol. 209 (1), pp. 213-23. Date of Electronic Publication: 2007 Oct 04. |
| DOI: |
10.1016/j.expneurol.2007.09.022 |
| Abstrakt: |
Growth factors such as glial cell line-derived neurotrophic factor (GDNF) have been shown to prevent neurodegeneration and promote regeneration in many animal models of Parkinson's disease (PD). Insulin-like growth factor 1 (IGF-1) is also known to have neuroprotective effects in a number of disease models but has not been extensively studied in models of PD. We produced human neural progenitor cells (hNPC) releasing either GDNF or IGF-1 and transplanted them into a rat model of PD. hNPC secreting either GDNF or IGF-1 were shown to significantly reduce amphetamine-induced rotational asymmetry and dopamine neuron loss when transplanted 7 days after a 6-hydroxydopamine (6-OHDA) lesion. Neither untransduced hNPC nor a sham transplant had this effect suggesting GDNF and IGF-1 release was required. Interestingly, GDNF, but not IGF-1, was able to protect or regenerate tyrosine hydroxylase-positive fibers in the striatum. In contrast, IGF-1, but not GDNF, significantly increased the overall survival of hNPC both in vitro and following transplantation. This suggests a dual role of IGF-1 to both increase hNPC survival after transplantation and exert trophic effects on degenerating dopamine neurons in this rat model of PD. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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