Rapid detection of ESBL-producing Klebsiella pneumoniae in blood cultures by fluorescent in-situ hybridization.

Autor: Palasubramaniam S; Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia., Muniandy S, Navaratnam P
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2008 Jan; Vol. 72 (1), pp. 107-9. Date of Electronic Publication: 2007 Dec 03.
DOI: 10.1016/j.mimet.2007.10.008
Abstrakt: Multi-resistant Enterobacteriaceae pose a serious threat of hospital acquired infections and their rapid identification is important for better clinical outcome. This study describes the rapid identification of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae of the sulphydryl variable-type by fluorescent in-situ hybridization. The method which rapidly identifies the target genes within 1 h could be a potentially rapid bacterial diagnostic tool.
Databáze: MEDLINE