Rapid electrodialytic clean-up of biological samples for high-performance liquid chromatography.

Autor: Brewster JD; US Department of Agriculture, ARS, Eastern Regional Research Center, Philadelphia, PA 19118., Piotrowski EG
Jazyk: angličtina
Zdroj: Journal of chromatography [J Chromatogr] 1991 Nov 01; Vol. 585 (2), pp. 213-7.
DOI: 10.1016/0021-9673(91)85079-u
Abstrakt: A sample clean-up system employing electrodialysis with size-selective and charge-selective membranes is described. When applied to the treatment of 0.5-ml milk samples containing sulfamethazine, the system produced an undiluted, clear solution in 3 min and eliminated the components in untreated milk that caused column fouling and double peaks. In contrast to conventional liquid- and solid-phase extraction procedures, electrodialytic clean-up is readily automated and uses no organic solvents.
Databáze: MEDLINE