| Autor: |
Nikolaenko IV, Goncharenko VS, Shimko NN, Galkin AIu |
| Jazyk: |
ruština |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2007 Mar-Apr; Vol. 79 (2), pp. 114-22. |
| Abstrakt: |
The scheme of immunoaffinity isolation of surface antigen of hepatitis B virus is developed. Selection of monoclonal antibodies was carried out and conditions for synthesis of immunoaffinity matrix were optimized; specificity of binding of HBs-antigen with a sorbent is improved; conditions of washing of columns and elution of HBs-antigen are chosen. The yield of purified HBs-antigen obtained according to the developed scheme exceeded 90% and it allows to use such HBsAg for immunization of animals and application in immunoanalysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
