Autor: |
Gomes-Figueiredo J; Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Patologia Básica, Centro Politécnico s/n, PO Box 19031 - 81531-990, Curitiba, PR - Brazil., Pimentel IC, Vicente VA, Pie MR, Kava-Cordeiro V, Galli-Terasawa L, Pereira JO, de Souza AQ, Glienke C |
Jazyk: |
angličtina |
Zdroj: |
Canadian journal of microbiology [Can J Microbiol] 2007 Oct; Vol. 53 (10), pp. 1123-32. |
DOI: |
10.1139/W07-078 |
Abstrakt: |
Thirteen endophytic fungal strains of the genus Pestalotiopsis were isolated from the medicinal plant Maytenus ilicifolia Mart. ex. Reiss (commonly known as "espinheira santa") and their antimicrobial properties were investigated. Two isolates were successful in inhibiting the growth of the tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA)) using the technique of bioautographic thin-layer chromatography (TLC) agar overlay assay. An analysis based on a polyphasic approach integrating taxonomic information, morphological traits, RAPD markers, and the sequencing of the ITS1-5.8S-ITS2 of the rDNA led to the assignment of the isolates as belonging to the species Pestalotiopsis microspora, Pestalotiopsis vismiae, and Pestalotiopsis leucothoes. Therefore, the present study presents a new approach to the study of endophytic fungi of the genus Pestalotiopsis. |
Databáze: |
MEDLINE |
Externí odkaz: |
|