Autor: |
Canter Cremers HC; Department of Plant Molecular Biology, Leiden University, The Netherlands., Stevens K, Lugtenberg BJ, Wijffelman CA, Batley M, Redmond JW, Breedveld MW, Zevenhuizen LP |
Jazyk: |
angličtina |
Zdroj: |
Carbohydrate research [Carbohydr Res] 1991 Sep 30; Vol. 218, pp. 185-200. |
DOI: |
10.1016/0008-6215(91)84097-x |
Abstrakt: |
The exopolysaccharide from R. leguminosarum bv. viciae strain 248 differs from those of other Rhizobium strains with similar symbiotic behavior. 13C-N.m.r. spectroscopy of fragments generated by partial hydrolysis, together with methylation analysis and 13C-n.m.r. spectroscopy of the enzymically depolymerised exopolysaccharide, indicated the following nonasaccharide repeating-unit: [formula: see text] The locations of the acetyl and 3-hydroxybutanoyl substituents in the exopolysaccharide are assigned provisionally. R. leguminosarum bv. viciae strain 248, cured of its Sym plasmid pRL1JI, synthesised an exopolysaccharide in which the sites and degree of substitution were unchanged. A Tn5 mutant, derived from strain 248 and unable to induce nodules, synthesised small amounts of EPS that lacked galactose. |
Databáze: |
MEDLINE |
Externí odkaz: |
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