| Autor: |
Chepurwar SB; Department of Pharmaceutical Analytical Chemistry, R.C. Patel College of Pharmacy, Shirpur, Dist-Dhule, Maharashtra State, India., Shirkhedkar AA, Bari SB, Fursule RA, Surana SJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatographic science [J Chromatogr Sci] 2007 Sep; Vol. 45 (8), pp. 531-6. |
| DOI: |
10.1093/chromsci/45.8.531 |
| Abstrakt: |
A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method is described for the simultaneous determination of levofloxacin hemihydrate and ornidazole in tablet dosage form. The method is based on the HPTLC separation of the two drugs followed by densitometric measurements of their spots at 298 nm. The separation is carried out on Merck TLC aluminium sheets of silica gel 60 F254 using n-butanol-methanol-ammonia (5:1:1.5, v/v/v) as mobile phase. The linearity is found to be in the range of 50-250 and 100-500 ng/spot for levofloxacin hemihydrate and ornidazole, respectively. The method is successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients are found. The suitability of this HPTLC method for the quantitative determination of the compounds is proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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