| Autor: |
Ekena JL; University of Wisconsin, Madison, 587 Medical Science Center, 1300 University Avenue, Madison, WI 53706, USA., Stanton BC, Schiebe-Owens JA, Hull CM |
| Jazyk: |
angličtina |
| Zdroj: |
Eukaryotic cell [Eukaryot Cell] 2008 Jan; Vol. 7 (1), pp. 49-57. Date of Electronic Publication: 2007 Nov 09. |
| DOI: |
10.1128/EC.00377-07 |
| Abstrakt: |
Sexual development in the human fungal pathogen Cryptococcus neoformans is a multistep process that results in the formation of spores, the likely infectious particles. A critical step in this developmental process is the transition from bud-form growth to filamentous growth. This transition is controlled by the homeodomain transcription factors Sxi1alpha and Sxi2a, whose targets are largely unknown. Here we describe the discovery of a gene, CLP1, that is regulated by Sxi1alpha and Sxi2a and is essential for sexual development. In vitro binding studies also show that the CLP1 promoter is bound directly by Sxi1alpha and Sxi2a. The deletion of CLP1 leads to a block in sexual development after cell fusion but before filament formation, and cells without CLP1 are unable to grow vegetatively after cell fusion. Our findings lead to a model in which CLP1 is a downstream target of the Sxi proteins that functions to promote growth after mating and to establish the filamentous state, a critical step in the production of spores. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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