| Autor: |
Huan LF; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. lifanghuan99@hotmail.com, Yao LH, Chen AJ, Cheng CS, Jia RQ, Tian Q, Bo H, Guo JQ, Wang M, Shu YL, Zhang ZQ |
| Jazyk: |
čínština |
| Zdroj: |
Bing du xue bao = Chinese journal of virology [Bing Du Xue Bao] 2007 Sep; Vol. 23 (5), pp. 366-70. |
| Abstrakt: |
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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