[Solubilization of components of outer membrane of Leptospira interrogans serovar mozdok by sodium desoxycholate and triton X-100 and evaluation of their immunogenic capacity and homologous and heterologous protection induced in hamsters].

Autor: Rivera YF; Laboratorio de Bioquímica, Instituto Finlay. yofernandez@finlay.edu.cu, Navarro YP, Mazorra YA, Rodríguez AG, Padrón Mde L, Valdés Y
Jazyk: Spanish; Castilian
Zdroj: Revista cubana de medicina tropical [Rev Cubana Med Trop] 2005 Jan-Apr; Vol. 57 (1), pp. 53-4.
Abstrakt: Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on applying the treatment with triton X-100. The variants formulated starting from the extracts obtained showed a high immunogenicity and capacity of homologous protection against the challenge in hamster.
Databáze: MEDLINE