Enlightenment about the new Architect-i2000 estradiol (Abbott Laboratories) immunoassay during in vitro fertilization.

Autor: Taieb J; Department of Biochemistry and Hormonology, AP-HP, Hospital Antoine Béclère, Clamart, France. joelle.taieb@abc.aphp.fr, Mendez Lozano DH, Benattar C, Messaoudi C, Poüs C
Jazyk: angličtina
Zdroj: Clinical biochemistry [Clin Biochem] 2007 Dec; Vol. 40 (18), pp. 1423-6. Date of Electronic Publication: 2007 Sep 25.
DOI: 10.1016/j.clinbiochem.2007.09.009
Abstrakt: Objective: We assessed a new estradiol (E2) immunoassay on the Architect-i2000 (Abbott Laboratories) for monitoring ovulation stimulation for IVF-ET and re-establishing clinical cut-off points. The method has been modified to improve E2 measurements especially at normal and low concentrations.
Design and Method: E2 was determined for 552 samples, from 83 women, presenting normal follicular status and undergoing 100 cycles of IVF treatment. We assessed the value of this assay for down-regulation of E2 concentration limit using gonadoliberin-releasing hormone agonist (GnRHa), and monitoring of the ovarian hyperstimulation, expected range of E2 per mature follicle prior to the administration of exogenous hCG and day 3 concentration limit. We compared results with our routine method (E2-6II Advia-Centaur; Siemens-Diagnostics) for which decision-making values were known.
Results: Considering E2 concentrations obtained with the new Architect-i2000 assay for patients treated with GnRHa for 2 weeks, the cutoff-point for ovarian down-regulation should be set down at 110 pmol/L to maintain 100% of sensitivity. Considering day 3 concentration limit determination, results were not significantly different from those obtained with our routine method. The mean E2 values per mature follicle fell into the range generally expected.
Conclusion: E2 determination with the new E2 Architect-i2000 assay could be used to monitor ovulation, in patients undergoing IVF-ET, in combination with transvaginal ultrasound.
Databáze: MEDLINE
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