[Production of monoclonal antibodies to staphylococcal alpha-toxin using the technique of in vitro immunization].

Autor: Beliaev NN, Zakir'ianova GK, Kostin GO, Tleulieva RT, Agashkin AO, Navasardiants DG, Ezepchuk IuV, Beklemishev AB
Jazyk: ruština
Zdroj: Biulleten' eksperimental'noi biologii i meditsiny [Biull Eksp Biol Med] 1991 Jul; Vol. 112 (7), pp. 73-6.
Abstrakt: The aim of this study is production of monoclonal antibodies (MAB) to staphylococcal alpha-toxin (SAT). SAT was obtained from culture medium of S. aureus s. 0-15 with ion-exchange chromatography and chromatofocusing. SAT was conjugated with CH-sepharose 4B and used for in vitro immunization of spleen cells, extracted from intact BALB/c mouse.
Databáze: MEDLINE