| Autor: |
Bezuglyĭ SV, Babichev VV, Skripal' IG, Malinovskaia LP |
| Jazyk: |
ruština |
| Zdroj: |
Mikrobiologicheskii zhurnal [Mikrobiol Zh (1978)] 1991 Sep-Oct; Vol. 53 (5), pp. 21-6. |
| Abstrakt: |
Two forms of DNA-dependent DNA-polymerase have been isolated and partially purified from the limited amount of biomass of cells Acholeplasma laidlawii PG-8, a typical representative of genus Acholeplasmataceae, as a result of successive chromatography on the columns with DEAE-cellulose DE-52 and Green A-sepharose. The first form of DNA-polymerase is eluted from the ion-exchange column with NaCl concentration of 0.1 M from the column with Green A-sepharose of 0.27 M, while the second form-with NaCl concentrations of 0.6 and 0.4 M, respectively. The both enzymatic activities are able to implement DNA synthesis. The conditions of DNA-polymerase production proved to be rather convenient for isolation of the concentrated and highly active enzymes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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