Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron.

Autor: Hardy ME; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah, USA., Ross LV, Chien CB
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2007 Nov; Vol. 236 (11), pp. 3071-6.
DOI: 10.1002/dvdy.21318
Abstrakt: Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression.
(Copyright 2007 Wiley-Liss, Inc.)
Databáze: MEDLINE
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