| Autor: |
Steindel M; Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, 88040-900, SC, Brazil., Kramer Pacheco L, Scholl D, Soares M, de Moraes MH, Eger I, Kosmann C, Sincero TC, Stoco PH, Murta SM, de Carvalho-Pinto CJ, Grisard EC |
| Jazyk: |
angličtina |
| Zdroj: |
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2008 Jan; Vol. 60 (1), pp. 25-32. Date of Electronic Publication: 2007 Sep 24. |
| DOI: |
10.1016/j.diagmicrobio.2007.07.016 |
| Abstrakt: |
During March 2005, 24 cases of acute human Chagas disease were detected in Santa Catarina State, southern Brazil, all of them related to the ingestion of Trypanosoma cruzi-contaminated sugar cane juice. Following field studies allowed the isolation of 13 T. cruzi strains from humans, opossums (Didelphis aurita and Didelphis albiventris), and vectors (Triatoma tibiamaculata). The isolated strains were characterized by multilocus enzyme electrophoresis (MLEE) and analysis of the spliced-leader and 24Salpha rRNA genes. The assays revealed that all strains isolated from humans belong to the TcII group but revealed a TcII variant pattern for the phosphoglucomutase enzyme. Strains isolated from opossums also showed a TcI profile in all analysis, but strains isolated from triatomines revealed a mixed TcI/TcII profile by MLEE. No indication of the presence of Trypanosoma rangeli was observed in any assay. Considering that mixed strains (TcI/TcII) were isolated from triatomines in an area without active vectorial transmission to humans and that all strains isolated from humans belong to the TcII group, our results show that T. cruzi TcI and TcII groups are circulating among reservoirs and vectors in southern Brazil and indicate that selection toward TcII group in humans may occur after ingestion of a mixed (TcI/TcII) T. cruzi population. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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