| Autor: |
Amoudy HA; Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait. hanady@hsc.edu.kw, Ahmad S, Thole JE, Mustafa AS |
| Jazyk: |
angličtina |
| Zdroj: |
Scandinavian journal of immunology [Scand J Immunol] 2007 Oct; Vol. 66 (4), pp. 422-5. |
| DOI: |
10.1111/j.1365-3083.2007.01961.x |
| Abstrakt: |
Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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