| Autor: |
Nowacki TM; Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA., Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M |
| Jazyk: |
angličtina |
| Zdroj: |
Cellular immunology [Cell Immunol] 2007 May; Vol. 247 (1), pp. 36-48. Date of Electronic Publication: 2007 Sep 07. |
| DOI: |
10.1016/j.cellimm.2007.07.004 |
| Abstrakt: |
For immune diagnostic purposes it would be critical to be able to distinguish between ongoing immune processes, such as active infections, and long-term immune memory, for example imprinted by infections that have been cleared a long time ago or by vaccinations. We tested the hypothesis that the secretion of granzyme B, as detected in ex vivo ELISPOT assays, permits this distinction. We studied EBV-, flu- and CMV-specific CD8(+) cells in healthy individuals, Vaccinia virus-reactive CD8(+) cells in the course of vaccination, and HIV-specific CD8(+) cells in HIV-infected individuals. Antigen-specific ex vivo GzB production was detected only transiently after Vaccinia immunization, and in HIV-infected individuals. Our data suggest that ex vivo ELISPOT measurements of granzyme B permit the identification of actively ongoing CD8(+) cell responses-a notion that is pertinent to the immune diagnostic of infections, transplantation, allergies, autoimmune diseases, tumors and vaccine development. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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