| Autor: |
Ludwig SR, Bowen B, Beach L, Wessler SR |
| Jazyk: |
angličtina |
| Zdroj: |
Science (New York, N.Y.) [Science] 1990 Jan 26; Vol. 247 (4941), pp. 449-50. |
| DOI: |
10.1126/science.247.4941.449 |
| Abstrakt: |
The temporal and spatial patterns of anthocyanin pigmentation in the maize plant are determined by the presence or absence of the R protein product, a presumed transcriptional activator. At least 50 unique patterns of pigmentation, conditioned by members of the R gene family, have been described. In this study, microprojectiles were used to introduce into maize cells a vector containing the transcription unit from one of these genes (Lc) fused to a constitutive promoter. This chimeric gene induces cell autonomous pigmentation in tissues that are not normally pigmented by the Lc gene. As a reporter for gene expression studies in maize, R is unique because it can be quantified in living tissue simply by counting the number of pigmented cells following bombardment. R may also be useful as a visible marker for selecting stably transformed cell lineages that can give rise to transgenic plants. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
