| Autor: |
Schoep TD; Murdoch University, Western Australian State Agricultural Biotechnology Centre (SABC), School of Biological Sciences and Biotechnology, South St, Murdoch, 6150 Perth, Australia., Gregg K; Curtin University, Biomedical Sciences, Kent Street, Bentley, 6845 Perth, Australia. |
| Jazyk: |
angličtina |
| Zdroj: |
Microbiology (Reading, England) [Microbiology (Reading)] 2007 Sep; Vol. 153 (Pt 9), pp. 3071-3080. |
| DOI: |
10.1099/mic.0.2007/006502-0 |
| Abstrakt: |
Novel plasmids were constructed for the analysis of DNA fragments from the rumen bacterium Pseudobutyrivibrio ruminis. Five previously unidentified promoters were characterized using a novel primer extension method to identify transcription start sites. The genes downstream of these promoters were not identified, and their activity in expression of genomic traits in wild-type P. ruminis remains putative. Comparison with promoters from this and closely related species revealed a consensus sequence resembling the binding motif for the RNA polymerase sigma(70)-like factor complex. Consensus -35 and -10 sequences within these elements were TTGACA and ATAATATA respectively, interspaced by 15-16 bp. The consensus for the -10 element was extended by one nucleotide upstream and downstream of the standard hexamer (indicated in bold). Promoter strengths were measured by reverse transcription quantitative PCR and beta-glucuronidase assays. No correlation was found between the composition and context of elements within P. ruminis promoters, and promoter strength. However, a mutation within the -35 element of one promoter revealed that transcriptional strength and choice of transcription start site were sensitive to this single nucleotide change. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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