| Autor: |
Wettey FR; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK., Jackson AP |
| Jazyk: |
angličtina |
| Zdroj: |
Sub-cellular biochemistry [Subcell Biochem] 2006; Vol. 40, pp. 119-43. |
| DOI: |
10.1007/978-1-4020-4896-8_9 |
| Abstrakt: |
Clathrin-coated pits and vesicles play a major role in eukaryotic membrane trafficking pathways. We have used the DT40 system to delete endogenous clathrin genes selectively from DT40 and replace them with clathrin under the control of a tetracycline-regulatable promoter. This enabled clathrin expression to be manipulated, and the functional consequences of clathrin depletion to be studied in a stable vertebrate context. Here we describe the background to the work on clathrin, our practical experience with using the tetracycline-regulatable expression system in DT40 and some novel insights into membrane trafficking pathways obtained using the cell-lines generated during the course of this work. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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