| Autor: |
Schuurman T; Department of Research and Development, Laboratory for Infectious Diseases, Groningen, The Netherlands., Roovers A, van der Zwaluw WK, van Zwet AA, Sabbe LJ, Kooistra-Smid AM, van Duynhoven YT |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of microbiological methods [J Microbiol Methods] 2007 Sep; Vol. 70 (3), pp. 406-15. Date of Electronic Publication: 2007 Jun 02. |
| DOI: |
10.1016/j.mimet.2007.05.016 |
| Abstrakt: |
5'-Nuclease and a hybridization probe assays for the detection of shiga toxin-producing Escherichia coli were validated with regard to selectivity, analytical sensitivity, reproducibility and clinical performance. Both assays were capable of detecting the classical stx(1) and stx(2) genes when challenged with reference strains of E. coli (n=40), although 1 to 4 minority sequence variants, whose clinical relevance is limited (stx(1c), stx(1d), and stx(2f)), were detected less efficiently or not at all by one or both assays. No cross reaction was observed for both assays with 37 strains representing other gastrointestinal pathogens, or normal gastrointestinal flora. Analytical sensitivity ranged from 3.07 to 3.52 log(10) and 3.42 to 4.63 log(10) CFU/g of stool for 5'-nuclease and hybridization probe assay, respectively. Reproducibility was high with coefficients of variation of
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| Databáze: |
MEDLINE |
| Externí odkaz: |
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