| Autor: |
Silove M; Department of General Physiology, School of Dentistry, University of the Witwatersrand, Johannesburg, South Africa., Ritchie AL, Hattingh J |
| Jazyk: |
angličtina |
| Zdroj: |
General pharmacology [Gen Pharmacol] 1991; Vol. 22 (5), pp. 883-7. |
| DOI: |
10.1016/0306-3623(91)90225-u |
| Abstrakt: |
1. Adrenaline (A) supplementation of the incubation medium of monolayer cultures of hepatocytes at 0.1, 0.2, 0.3, 1 and 10 ng/ml resulted in consistently enhanced levels of secreted and newly synthesised non-secreted proteins; supplementation with 100 and 1000 ng/ml resulted in lower or unchanged levels. These effects were most consistent 6 hr after a medium change. 2. Noradrenaline supplementation of the medium resulted in increased levels of secreted and non-secreted proteins at low concentrations (less than 2.4 ng/ml) at 6 hr after a medium change, but significantly decreased levels of both populations at high concentrations of noradrenaline (NA) (2.4 less than NA less than 1000 ng/ml), at 6-9 hr after a medium change and sustained to 24 hr, the most significant decrease being at 10 ng/ml. 3. All combinations of concentrations of A + NA resulted in non-dose-dependent decreased levels of both the secreted and non-secreted soluble protein fractions. The most significant decreases occurred at concentrations of (1 + 5) and (10 + 10) ng/ml adrenaline + noradrenaline. 4. Medium supplementation with adrenaline, noradrenaline or a combination of the two had no effect on the uptake of [3H]leucine by the cells. 5. The results are discussed in relation to receptor status on the hepatocyte membranes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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